mouse anti tsp 1  (Santa Cruz Biotechnology)

Journal: International Journal of Medical Sciences

Article Title: Thrombospondin-1 Gene Deficiency Worsens the Neurological Outcomes of Traumatic Brain Injury in Mice

doi: 10.7150/ijms.18812

Figure Lengend Snippet: Effects of TSP-1 knockout on BBB leakage and brain lesion in mice after TBI. To examine the contribution of TSP-1 in the outcomes of TBI, we first measured the TSP-1 protein expression changes in response to TBI, then measured the BBB leakage and brain lesion volumes in TSP-1 KO mice and WT mice. (A) Representative Western Blot image of TSP-1 protein levels in the ipsilateral hemisphere at 6 h, 1d, 3d, 5d, 7d, 10d, 14d, 21d following TBI. Samples from TSP-1 KO mice was used as negative control (n=4 for each time point). (B) BBB leakage was measured and quantified at 24 hrs after TBI by testing Evans Blue extravasation (n=4, *p<0.05 vs WT group). (C) Brain lesion volume was measured and quantified at 28 days after TBI (n=16).

Article Snippet: Three different antibodies for TSP-1 (mouse anti-TSP-1, NeoMarkers; mouse anti-TSP-1, Santa Cruz; rabbit anti-TSP-1, Abcam) were used to ensure the validity of Western blot.

Techniques: Knock-Out, Expressing, Western Blot, Negative Control

Journal: International Journal of Medical Sciences

Article Title: Thrombospondin-1 Gene Deficiency Worsens the Neurological Outcomes of Traumatic Brain Injury in Mice

doi: 10.7150/ijms.18812

Figure Lengend Snippet: Effect of TSP-1 knockout on behavior outcomes in mice after TBI. Neurological function outcomes were measured after TBI in TSP-1 KO and WT mice. Motor-sensor functions including NSS, wire gripping and corner test were assessed before and 1d, 3d, 5d, 7d, 10d, 14d, 21d after TBI. (A) NSS test; (B) wire gripping test; (C) corner test. Moreover, spatial memory ability was assessed by Morris Water Maze starting from 14d post-TBI. (D) latency training; (E) probe trials measuring the entry times to platform; (F) probe trials measuring the stay time in targeted quadrant. (n=16, * p<0.05 vs WT group).

Article Snippet: Three different antibodies for TSP-1 (mouse anti-TSP-1, NeoMarkers; mouse anti-TSP-1, Santa Cruz; rabbit anti-TSP-1, Abcam) were used to ensure the validity of Western blot.

Techniques: Knock-Out

Journal: International Journal of Medical Sciences

Article Title: Thrombospondin-1 Gene Deficiency Worsens the Neurological Outcomes of Traumatic Brain Injury in Mice

doi: 10.7150/ijms.18812

Figure Lengend Snippet: Effect of TSP-1 knockout on synaptogenic responses in mouse brains after TBI. Synaptogenic response was examined by testing synaptophysin and BDNF protein levels in bilateral hemispheres by Western blot at 21 days after TBI. (A) Representative Western blot images for synaptophysin and BDNF in sham and injured brain tissue (n=4/group). (B) Quantification of synaptophysin protein levels showed significant decrease in the contralateral hemisphere of TSP-1 KO mice after TBI, compared with WT. (C) Quantification of BDNF protein level showed significant decrease in the contralateral hemisphere of TSP-1 KO mice compared to WT after TBI, and significant elevation in the ipsilateral hemisphere of both groups compared to sham after TBI. (# p<0.05 vs sham, *p<0.05 vs WT group).

Article Snippet: Three different antibodies for TSP-1 (mouse anti-TSP-1, NeoMarkers; mouse anti-TSP-1, Santa Cruz; rabbit anti-TSP-1, Abcam) were used to ensure the validity of Western blot.

Techniques: Knock-Out, Western Blot

Journal: International Journal of Medical Sciences

Article Title: Thrombospondin-1 Gene Deficiency Worsens the Neurological Outcomes of Traumatic Brain Injury in Mice

doi: 10.7150/ijms.18812

Figure Lengend Snippet: Effect of TSP-1 knockout on vessel density and vascular response in mouse brains after CCI. At 21 days after TBI, the brain vessel density in the peri-lesion cortex area was measured by immunostaining with anti-CD31 antibody. Vascular responses was measured by testing the protein expression of angiogenic factors VEGF and Ang-1. (A) Representative images of CD31-positive vessels in the ipsilateral peri-lesion cortex at 21 days after TBI or sham operation (Scale bar =50um). (B) Quantitative analysis of vessel density defined as the area fraction of positive signal. (n=4/group). (C) Representative images of Western blot for VEGF and Ang-1 protein levels in sham and injured hemispheres at 21 days after TBI (n=4/group). (D) Quantification of VEGF protein level showed no difference between WT and TSP-1 KO groups. (E) Quantification of Ang-1 protein level showed significant increase in the ipsilateral hemisphere of TSP-1 KO mice compared with WT mice, after TBI (n=4/group). (# p<0.05 vs sham, *p<0.05 vs WT group).

Article Snippet: Three different antibodies for TSP-1 (mouse anti-TSP-1, NeoMarkers; mouse anti-TSP-1, Santa Cruz; rabbit anti-TSP-1, Abcam) were used to ensure the validity of Western blot.

Techniques: Knock-Out, Immunostaining, Expressing, Western Blot